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Protein Sciences

Uniting Drug Development Expertise with Antibody & Protein Production Services

Mammalian Protein Production

Mammalian Expression and Purification Services

 

In the rapidly evolving field of protein production, mammalian cell expression systems have become the industry standard for creating complex, high-quality proteins. In the Protein Sciences (PS) Department, we use the high-yield expression systems of CHO and HEK293 cells in our transient expression services, providing you with speed, flexibility, and cost-effectiveness. This enables swift protein production for proof-of-concept studies and early-stage research.

 

In addition, we employ the same parental CHO cell line and NS0 cells for challenging protein production in our stable expression services. This ensures a seamless scale-up process and consistent yields for your large-scale protein production. Having produced over 3,500 non-antibody proteins using these mammalian cell lines, we are a trusted partner for a wide range of research and biologics drug development innovations.

Our services offer speed and cost-effectiveness for transient expression and seamless scale-up for stable expression using CHO system.

Transient Expression and Purification Services (HEK293 & CHO)

 

Utilizing our high-titer expression systems, we excel in high-throughput (HTP) transient expression and purification, employing both HEK293 and CHO cells. This allows for the rapid screening of a wide variety of molecules. Our extensive expertise encompasses the production of a diverse range of proteins, including cytokines, MHCs, TCRs, enzymes, transcription factors, as well as the extracellular domains (ECD) of cell surface receptors and membrane proteins.

Transient Expression (HEK293 & CHO) Workflow

Starting from gene synthesis, coning DNA prep, transient protein expression, protein purification and protein characterization can be completed in 3 weeks.

Key Features of our Transient Expression and Purification Services:

  • HTP small-scale protein production with daily throughput of 1,000 proteins
  • High-titer expression system for faster and more economical protein production
  • High-purity protein delivered in as little as 3 weeks

Transient Expression and Purification Service Details:

Service Item Deliverables Expression Scale Duration QC Request A Quote
Highly customizable package with multi-step purification Reagent protein, extracellular domain, membrane protein, antibody with clipping issue 1-50 L 3-5 weeks

<0.5 EU/mg endotoxin control, QC incl. Titer, A280, Caliper (R/NR) or SDS-PAGE, SEC-HPLC

 Request A Quote
Additional QC RP-HPLC, bioburden, DSF, DSC, SPR, LC-MS, SEC-MALS, peptide mapping, glycan profiling, cIEF, CHO HCP, residual DNA, ELISA, Western blot, freeze thaw study, micro developability testing

Case Study: A Solution to the Challenges in Protein Engineering: Our HTP Transient Expression Services

 

In the field of protein engineering, there is often a need to screen numerous protein constructs in order to identify those that demonstrate high expression levels without any aggregates or degradation. Cost is typically a crucial factor in such projects. We offer an ideal solution to tackle these challenges through our HTP transient expression services.

Figure A: 24 constructs were produced by cloning 12 different proteins with either a His tag or Fc tag. Subsequently, these constructs were expressed in HEK293 cells utilizing 24-deep well plates. All constructs were expressed and purified in a single batch, demonstrating a rapid and efficient method for screening a large number of constructs to evaluate their expression levels.

High throughput transient expression using 24-deep well plate for His-tag proteins and Fc-tag proteins.

Mammalian Stable Expression and Purification Services

 

Stable expression and purification play a pivotal role in projects that require sustained, dependable protein yields over time. Our platform harnesses both CHO and NS0 cell lines to fulfill these requirements. One significant advantage of utilizing our CHO cells lies in their lineage, originating from the same parental cell line as our transient CHO cells. This common origin facilitates smooth scale-up procedures. Moreover, this continuity extends seamlessly to CMC activities, establishing an integrated pathway from research to production. In cases where challenges arise with CHO cells, our NS0 line offers an alternative option, demonstrating robust performance across a wide spectrum of proteins.

Key Features of Our Mammalian Stable Expression and Purification Services:

  • Seamless Scale-up and CMC Progression: Utilize the same parental CHO cells for a smooth transition from research to manufacturing.
  • Quality Assurance: Expertise in producing a variety of high-quality proteins.
  • Fast Turnaround: Gram-level protein production in 6-7 weeks.

Mammalian Stable Expression and Purification CHO Services Details:

Service Item Deliverables Expression Scale Duration QC Request A Quote
Gram-level protein generation for animal study and developability study in CHO cells Any antibody, tagged or untagged protein with need for scale-up manufacturing 20 mL-50 L 6-7 weeks A280, Caliper (R/NR) or SDS-PAGE (R/NR),
SEC-HPLC, and endotoxin (<0.5 EU/mg)		 Request A Quote
Additional QC RP-HPLC, bioburden, DSF, DSC, SPR, LC-MS, SEC-MALS, peptide mapping, glycan profiling, cIEF, CHO HCP, residual DNA, ELISA, Western blot, freeze thaw study, micro developability testing

Mammalian Stable Expression and Purification NS0 Services Details:

Service Item Deliverables Duration QC Request A Quote
1-50 L Challenge protein or cystine rich protein production 6-7 weeks

<0.5 EU/mg endotoxin control, QC incl. Titer, A280, Caliper (R/NR) or SDS-PAGE, SEC-HPLC

 Request A Quote
Additional QC RP-HPLC, bioburden, DSF, DSC, SPR, LC-MS, SEC-MALS, peptide mapping, glycan profiling, cIEF, CHO HCP, residual DNA, ELISA, Western blot, freeze thaw study, micro developability testing

Case Study: Proven Consistency & Reproducibility: WuXian™ Express Delivers Across All Scales

Figure A: These data illustrate remarkable consistency and reproducibility across various batches of protein production, ranging from 200 L to 2,000 L. Utilizing our CHO stable platform, WuXian™ Express, the production of the target protein in 3 distinct batches (one at 200 L and two at 2,000 L) not only showcased high consistency during the transition from medium to large scale, but also demonstrated high batch-to-batch reproducibility, as confirmed by SEC-HPLC, cIEF, SDS_CALIPER, and Glycosylation analysis.

Showcase high consistency and high batch-to-batch reproducibility with WuXian™ Express platform, as confirmed by SEC-HPLC, cIEF, SDS_CALIPER, and Glycosylation analysis.

Figure B: These data demonstrate a significant improvement in the expression of a cysteine-rich protein when utilizing NS0 cells. A 39 kDa Fc-fusion protein containing 10 cysteines exhibited aggregation and disulfide bond scrambling when expressed in CHO cells. Switching to NS0 cells resulted in a notable increase in both yield and quality. The target protein reached a purity level of 94.3% with correctly formed disulfide bonds.

Express Host CHO NSO
AC Yield(mg/L) 312 327(correct disulfide bonds confirmed)
SEC-HPLC & SDS-PAGE gel of AC eluate Significant improvement in the expression of a cysteine-rich protein when utilizing NS0 cells. Significant improvement in the expression of a cysteine-rich protein when utilizing NS0 cells.

 

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