The process involves addressing the three important questions during the research phase. How well is your molecule being expressed? How well does your molecule perform during purification and scale-up? And how stable is your molecule?
“We provide valuable insights and guidance to clients throughout the entire protein and antibody production process, enabling clients to avoid common pitfalls and overcome challenges,” says Dr. Wu. “Our comprehensive end-to-end approach to protein production helps ensure a seamless and successful journey from concept to market.”
Offering solutions to overcome protein production challenges
Recently, a client came to PS with concerns about the stability of an enzyme-fused antibody. Teams within the department produced the client’s protein, but there were degradation issues that resulted in poor quality. To solve the issue, the teams used Mass spectrometry to locate the cleavage site. They also advised on how to solve low productivity issues by optimizing the protein’s construct. The solutions helped the enzyme survive in harsh production conditions.
“After a year of work, the client’s molecule is now ready for CMC,” says Dr. Wu. “The protein has no cleaving, good stability, and we increased its expression level by more than 30-fold. This is what separates WuXi Biologics from other CROs. We produce proteins in the context of knowing what’s feasible to progress to CMC in a high-quality, efficient, and cost-effective manner.”